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Promega flexi-cloning blend restriction enzymes
Flexi Cloning Blend Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flexi-cloning+blend+restriction+enzymes/flexi+cloning+blend+restriction+enzymes/pm26309236-54-27-31
Average 90 stars, based on 1 article reviews
flexi-cloning blend restriction enzymes - by Bioz Stars, 2026-11
90/100 stars

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Amplification:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).

Expressing:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).

Plasmid Preparation:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).

Binding Assay:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).

Modification:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).

Introduce:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).

Mutagenesis:

Article Title: Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
Article Snippet: The amplified gene product and pVP68K, an expression plasmid that fuses maltose binding protein (MBP) to the N-terminus of a target protein (14, 15), were digested with Flexi-cloning blend restriction enzymes (Promega, Madison WI) and ligated with T4 DNA ligase (Promega, Madison, WI), creating expression plasmid pVP68KT4moF (Figure S3). pVP68KT4moF was further modified to introduce two mutations, Lys270Ser and Lys271Ser, using QuikChange sitedirected mutagenesis (Stratagene, Santa Clara, CA).



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Promega flexi-cloning blend restriction enzymes
Flexi Cloning Blend Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flexi-cloning+blend+restriction+enzymes/flexi+cloning+blend+restriction+enzymes/pm26309236-54-27-31
Average 90 stars, based on 1 article reviews
flexi-cloning blend restriction enzymes - by Bioz Stars, 2026-11
90/100 stars
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